Pulsed-Field Gel Electrophoresis (PFGE)
Pulsed-Field Gel Electrophoresis (PFGE) is considered by many to be the "gold standard" method of molecular/genetic sub-typing of microorganisms. PFGE has typically proven superior to other methods including biochemical and molecular typing methods. PFGE has become the preferred tool for differentiating between isolates belonging to the same or closely related species and for monitoring and tracking the movement of specific strains in the environment. This highly discriminatory test creates a very specific, often distinct, DNA fragment pattern for each microbial isolate. Much like fingerprints, the DNA banding patterns prove useful to differentiate between isolates of a given microbial species. The DNA photographic profile (fingerprint) of each isolate generated is stored electronically and compared to a database of isolates using state-of-the-art analytical software. Please view our Sample PFGE Report (PDF) for more information. |